Livebirth after a Zona-Free ICSI of a Vitrified/Warmed Oocyte after Social Freezing Running Title: Is it Worth to use Zona-Free Oocytes for ICSI?

Normal development of a zona-free oocyte to the blastocyst stage following ICSI.

Laser-assisted zona pellucida thinning prior to routine ICSI.

BACKGROUND In MII oocytes showing difficult oolemma breakage, ICSI can cause an increase in the degeneration rate. This may be overcome by laser-assisted ICSI using a 5-10 micro m opening in the zona pellucida for injection. However, such a small opening might impair the hatching process, especially if assisted hatching is applied in addition. In order to prevent this, the present study used ro...

Rescue ICSI of unfertilized oocytes after IVF.

BACKGROUND Failed fertilization after IVF occurs in 10-20% of cycles. Conflicting results of rescue fertilization by ICSI have been reported. We therefore compared the success rate in terms of fertilization and pregnancy of cycles in which rescue ICSI was performed with those from a matched control group of primarily ICSI cycles. METHODS Unfertilized oocytes from IVF cycles with total fertili...

Case report: pregnancy outcome following ICSI of oocytes with abnormal cytoplasm and zona pellucida.

A couple with unexplained infertility was referred for routine IVF and ICSI treatment. Ovulation was induced by the GnRH analogue protocol combined with HMG and HCG. Preparation of denuded oocytes revealed a major disorder of the zona pellucida and abnormal oocytes. During preparation of ova for ICSI, 15 retrieved oocytes were denuded, 14 of which underwent ICSI treatment. Four of the oocytes c...

P-97: Optimized Combined Electrical-Chemical Parthenogenetic Activation for Zona Free Sheep Oocyte

Background: Artificial oocyte activation (AOA) has important implications in assisted reproductive technique (ART) with particular importance in somatic cell nuclear transfer (SCNT). Parthenogenesis activation using zona- free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. Materials and Methods: To establis...